Recommended needle sizes and injection sites for various species are indicated. Safety studies of the intraperitoneal injection of e1a. Get a printable copy pdf file of the complete article 285k, or click on a page image. Pull back on the plunger to ensure negative pressure prior to injecting. Intraperitoneal injection in mice simple pharmacology and facts of medicines. Pdf intraperitoneal injection is a common technique that safely delivers a. Intraperitoneal ip injection in rats and mice sop page 2 of 6 70% isopropyl alcohol to disinfect top of multi. The aim of this technique is to administer material into the space surrounding the abdominal organs, avoiding injection directly into any organ. Results of intraperitoneal and oral administration of thymoquinone in mice are given in tables1a and 1b, respectively. Considering the risks and safety of intraperitoneal injections article pdf available in lab animal 454. Intraperitoneal injection is a common method of administering drugs to rodents.
For all species, many different routes are available for administration of substances. Intraperitoneal administration of a novel tatbdnf peptide. Subcutaneous compared with intraperitoneal ketaminexylazin. Errors in the technique of intraperitoneal injection of mice. Here we present an overview of the methods used to deliver tamoxifen to mice.
Tail handling and scruff handling of mice one hand only duration. Evaluation of a novel technique for intraperitoneal injections in mice article pdf available in lab animal 4411. In this study, we performed intraperitoneal glucose tolerance tests in mice with dietinduced obesity and their lean controls, with glucose doses based on either the total body weight or the lean body mass of the animals. At 24 h after injection, the intensity of free dye was 100 folds less than the dir. Administration of substances to laboratory animals. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. The drug concentrations in peritoneal solid cancer tissue and suspended free cancer cells were also significantly higher for a longer time in the intraperitoneal injection group than in the intravenous injection group.
Good practice administration routes and volumes for mice. Intraperitoneal xenografts of human epithelial ovarian cancer in nude mice bruce g. In addition, fewer female mice achieved surgical anesthesia, suggesting a narrow therapeutic window for intraperitoneal kx in female mice. Intraperitoneal injection synonyms, intraperitoneal. Some sites, such as footpad injection of freunds complete adjuvant, intrasplenic injection and intra lymph node injection. Pharmacokinetic advantage of intraperitoneal injection of. Lactate treatment has shown a therapeutic potential for several neurological diseases, including alzheimers disease. Method of intraperitoneal injection in wistar rat duration. Intraperitoneal injection is a common technique that safely delivers a substance into the peritoneal cavity but can induce high stress in animals. Synonyms for intraperitoneal injection in free thesaurus. Effects of administration route, dietary condition, and blood. Many operators recommend restraining the animal in a head down, or trendelenburg, position, assuring that the viscera will be displaced. Abbot japan, tokyo, japan and sacrificed by exsanguination from aortic transection. The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible.
Intraperitoneal injection ip, intravenous injection iv or anal. Understand the benefits and risks of intraperitoneal therapy for advanced ovarian cancer. The goals of the study were to find a safe intraperitoneal injection anesthesia protocol for mediumduration surgery in mice e. Because it allows administering a larger volume with more radioactivity, we have investigated this route and the possibility of using it to circumvent the volume constraint we earlier experienced with pretargeting radiotherapy. Effective in vivo gene modification in mouse tissue.
Intraperitoneal injection definition of intraperitoneal. Intraperitoneal ip injection in rats and mice sop ubc animal. To determine glucose tolerance, we determined the blood glucose auc during the glucose tolerance test. The intramuscular technique requires more skill than does subcutaneous. For purposes of control, lard, olive oil and other fatty materials free from added carcinogenic compounds were injected into rats and mice in the same way. A serious consequence associated with this technique, however, is the puncture of vital organs such. Oct 14, 2015 appswe and wild type appwt mice at 12 months of age were given a daily intraperitoneal injection with the tatbdnf peptide pep, 2. Improvised peritoneal dialysis in an 18monthold child with severe acute malnutrition and acute kidney injury a case report p02. Intraperitoneal injection improves the uptake of nanoparticle. Intraperitoneal definition of intraperitoneal by medical.
Blood lactate dynamics in awake and anaesthetized mice after. Burrows the research institute, the royal cancer hospital free, london. In conclusion, intraperitoneal injection of omt could beneficially decrease the cciinduced mechanical allodynia and thermal hyperalgesia, antagonize the effect of nmda, synergistic enhance the effect of mk801 and infenprodil, led to a marked decrease in nmda nr2b, perk and pcreb induced by cci in the spinal cord in mice. However, pristane also induces plasmacytomas and an erosive arthritis resembling rheumatoid arthritis in balbc mice, probably as a consequence of enhanced interleukin 6 production. In this study, we tested the ability of avidin, labeled through conjugation to radioactive biotin a b vitamin, to target intraperitoneal tumors. The ld 50 of thymoquinone in rats was determined to be 57. Only the tip of the needle should penetrate the abdominal wall, to prevent injection into the intestine waynforth, 1980. The final biodistribution pattern of nphdl 59fespio after intravenous and intraperitoneal application in mice looked similar. Intraperitoneal injection in the mouse procedures with care.
In this study, intraperitoneal ip injection was used to mimic the human administration route. The effect of the subcutaneous and intraperitoneal injection of lard, olive oil and other fatty materials in rats and mice h. Media in category intraperitoneal injections the following 9 files are in this category, out of 9 total. If there is negative pressure, proceed with the injection.
This is consistent with the trend of greater effect on the nervous system of. Pdf evaluation of a novel technique for intraperitoneal. Intraperitoneal ip injection in rats and mice sop page 4 of 6 mouse rat 6. Effect of intraperitoneal injection volume and antibody protein dose on the pharmacokinetics of intraperitoneally administered igg2ac murine monoclonal antibody in the rat1 jeffrey s. Procedure summary and goal describes procedure for the administration of fluids or compounds into the abdominal cavity. Using 165 mice, 24 % received the ip injection in a place other than the peritoneal cavity. Retinal degeneration induced in adult mice by a single. Edta was found to be nontoxic to the cell lines by.
Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence. Pharmacokinetic advantage of intraperitoneal injection of docetaxel in the treatment for peritoneal dissemination of cancer in mice. Effect of intraperitoneal or intracerebroventricular. Considering the risks and safety of intraperitoneal injections. Optimization of intraperitoneal injection anesthesia in mice. Restrain mice with their abdomen exposed uppermost and their head pointed. Calculation of glucose dose for intraperitoneal glucose.
In conclusion, surgical anesthesia of mice with subcutaneous kx k, 191. Conclusion these data indicate that a single intraperitoneal injection of ketamine at subanaesthetic and anaesthetic doses does not impair working memory, reference memory or neurodegeneration in adult mice, but an intermediate dose of ketamine produces transitory hyperlocomotion. Mice mus musculus are small rodents that breed and sexually mature quickly, making them perfectly suited to generating large animal colonies for biological research. Those trained persons listed on an approved animal care committee protocol performing the procedure.
Intraperitoneal ip injections should be made into the animals right abdominal quadrant so as to avoid penetrating the cecum on the left side. Mar 22, 2016 considering the risks and safety of intraperitoneal injections. The laboratory mouse handbook of experimental animals. The laboratory mouse handbook of experimental animals usp. In a previous study, 14 mice were exposed to swcnts through intravenous iv injection to determine whether there were any adverse effects caused by swcnts on the mouse brain and also to find a safe application dosage if possible.
Dec 01, 1994 intraperitoneal injection of pristane 2,6,10,14 tetramethylpentadecane is a standard technique for obtaining monoclonal antibodyenriched ascitic fluid. The icr newborn male mice were randomly selected for intraperitoneal injection of stz beyotime institute of biotechnology, shanghai, china. Four tumor models one ovarian, one lung, and two colon were established in nude mice by intraperitoneal injection of cultured cancer cells. Intraperitoneal xenografts of human epithelial ovarian. It is more often applied to animals than to humans. The injection site should be in the lower left quadrant of the abdomen because vital organs are absent from this area. Pdf evaluation of a novel technique for intraperitoneal injections. Good practice administration routes and volumes for mice, rats, and rabbits are indicated in table 1. Intraperitoneal injection is not always a suitable.
Seven days after the injection, mice were anesthetized with isoflurane forane. Data available for intraperitoneal injection of 70% or 100% ethanol in mice suggests 4560s 75th percentile to achieve lorr and 3. As compared to other mammalian species, mice are simple and inexpensive to maintain in the laboratory. The november 2016 issue of lab animal included an article by baek and colleagues 1 describing a novel technique for handling mice. The control group was injected with the same amount of pbs buffer. The intramuscular administration is not recommended, as the muscle of the mouse is too small. Intraperitoneal injection is a common route for parenteral administration of drugs in rodents. In contrast, the time to lorr is considerably longer with xylazineketamine, taking 6.
Free dir and healthy mice without colitis were served as control. As seen in the clinical journal of oncology nursing by ons connect. Evaluation of a novel technique for intraperitoneal. Using 165 mice, 24% received the ip injection in a place other than the peritoneal cavity. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services.
Intraperitoneal injection an overview sciencedirect topics. Intraperitoneal injection or ip injection is the injection of a substance into the peritoneum body cavity. Nelson from the laboratories of physiological chemistry, iowa state college, ames received for publication, may 5, 1932. The highest activity uptake was measured in the liver and spleen, followed by the femur, aorta, and in the peritoneal macrophages in the case of intraperitoneal injection. Induction of lupusassociated autoantibodies in balbc mice. Intraperitoneal injection in mice research at penn state. Avidin targeting of intraperitoneal tumor xenografts jnci. Sevenweekold mice received an intraperitoneal ip injection of vehicle physiological saline or enu at a dose of 50, 100, 200, 400, or 600 mgkg 5 mice per dose. All animal users performing ip injections in rodents must. Health, general cancer chemotherapy health aspects injections, intraperitoneal research intraperitoneal injections ovarian cancer drug therapy risk factors. Intraperitoneal injection the administration of material into the peritoneal cavity is frequently performed in mice. Feb 26, 2016 see more video protocols, please visit restrain the mouse by the scruff method.
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